Citômetro de fluxo CytoFLEX

O citómetro de fluxo CytoFLEX, a primeira introdução à plataforma CytoFLEX, fornece o desempenho necessário num sistema fácil de usar, permitindo que o utilizador se centre na ciência e não se preocupe com a instrumentação. A sua sensibilidade e resolução superiores em todas as configurações dão-lhe vantagem sobre outros sistemas de citometria com quatro vezes mais o seu tamanho.•

 
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Características

Excelente sensibilidade

Versatilidade da plataforma

Manutenção reduzida

Automação ativada

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Label-free Technology to Assess Protein Loadings on the Surface of Biological Sample
Advancing Unmixing Algorithms in Spectral Flow Cytometry
Introducing the CytoFLEX mosaic Detection Module, where spectral flow cytometry meets modularity Introduction to the CytoFLEX mosaic Spectral Detection Module, a modular upgrade for CytoFLEX S and LX flow cytometers that adds spectral flow cytometry capabilities. This module allows users to switch between conventional and spectral detection modes, enhancing flexibility for a wide range of experimental workflows. It is designed to improve data resolution, simplify spectral analysis, and support future-proofing of existing instruments. The module integrates with CytExpert for Spectral software and FluoroFinder’s panel design tools, offering a streamlined and user-friendly experience for designing and analyzing complex multicolor flow cytometry experiments.
Optimization of a B cell multicolor panel with CytoFLEX mosaic 88 Spectral Detection Module The document presents the development and validation of a 32-color spectral flow cytometry panel designed for detailed immunophenotyping of B cell subsets. It includes markers relevant to B cell activation, differentiation, and function, as well as additional markers for myeloid cells and chronic lymphocytic leukemia (CLL), enabling broad immune profiling. The panel was implemented on the CytoFLEX mosaic 88 spectral detection module and tested using preserved bone marrow and cryopreserved PBMCs from healthy donors. The study demonstrates that this panel is effective for extracting rich immunological data from limited sample volumes, making it a valuable tool for translational research and retrospective clinical sample analysis.
Advancements in MESF Beads for Enhanced Fluorescence Quantification in Flow Cytometry The document discusses advancements in the development of MESF (Molecules of Equivalent Soluble Fluorochrome) beads for improved fluorescence quantification in flow cytometry. It highlights the importance of MESF beads in standardizing fluorescence measurements across instruments and experiments. The research introduces new nano-sized MESF beads incorporating various fluorescent dyes such as Pacific Blue®, SuperNova, FITC, PE, and APC. These beads are designed to have low background fluorescence and known dye content, making them suitable for small particle flow cytometry. Preliminary testing using the CytoFLEX nano Flow Cytometer demonstrated their potential for accurate quantification of dye molecules per bead. The study establishes a reliable methodology for MESF value calculation and shows how these beads can be used to determine fluorescence detection limits in biological samples. Although not yet commercially available, the new MESF beads are expected to become essential tools for instrument calibration and assay standardization in advanced flow cytometry applications.

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