CytoFLEX nano Flow Cytometer

The CytoFLEX nano Flow Cytometer is the newest member of the CytoFLEX Platform.

Specifically designed for the analysis of nanoparticles, the CytoFLEX nano Flow Cytometer overcomes the limitations of traditional flow cytometry, giving researchers a sensitive, consistent and flexible solution that can help them advance their research.

The CytoFLEX nano Flow Cytometer is a pivotal development in flow cytometry that allows users to analyze extracellular vesicles (EVs) at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection and 5 side scatter channels, to deliver full characterization and open additional avenues for your nanoparticle research.

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CytoFLEX nano Flow Cytometer Features

Explore the full picture of your EV experiment with the CytoFLEX nano Flow Cytometer. This purpose-built flow cytometer provides greater sensitivity, consistent instrument performance, and flexibility to study your sample.


With an unparalleled size sensitivity from 1µm to 40nm* and 10nm resolution,** the CytoFLEX nano flow cytometer enables the detection of smaller EV populations and their low-abundance cargo.


Open up your research with 6 fluorescent channels and 5 side scatter channels. Gather all the necessary data with fewer constraints.

Maximum Consistency

Maintaining contamination-free, robust instrument performance is critical when working with small size particles. Obtain reproducible results you can trust with >90% volumetric counting accuracy and <1% carryover between samples thanks to automated QC controls.


Built on the same principles as the CytoFLEX platform, the CytoFLEX nano flow cytometer features a similar footprint and user-friendly software interface.


The CytoFLEX nano analyzer utilizes  CytExpert for CytoFLEX Acquisition and Analysis Software and has the ability to count, size, and characterize EVs using a single technique, propelling your research forward.

Size  Count  Characterize
Particle size determination by flow cytometry requires calibration using an appropriately characterized reference particle paired with a calibration method. Latex polystyrene particles are the tool of choice for this task due to their NIST-traceable nature and reliably consistent manufacturing. Through the calibration method taught to each CytoFLEX nano flow cytometer customer, data will be reliably reportable in absolute units for method comparison and correlation. The most robust counting methods rely on highly controlled volumetric delivery of samples rather than hand loading samples with the implied variability. The CytoFLEX nano flow cytometer allows users to customize their sample handling so that the right amount of sample is used for the results needed. The ability to describe nanoscale particles requires best-in-class sensitivity. To make this possible, the CytoFLEX nano flow cytometer has completely redesigned the optics and fluidics system to maximize the detection of even the smallest amounts of fluorescence across 6 detection channels and excited by 4 collinear lasers.

Performance Characteristics

CytoFLEX nano Flow Cytometer Performance
 Violet side scatter sensitivity VSSC1: 40 nm relative to polystyrene nanoparticles
VSSC2: 80 nm relative to polystyrene nanoparticles
Scatter detection dynamic range VSSC 1 for small range 40-150 nm polystyrene nanoparticles
VSSC2 for large range 80-1000 nm polystyrene nanoparticles
Violet forward scatter sensitivity 300 nm relative to polystyrene nanoparticles
Fluorescence sensitivity and resolution

 Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity Fluorospheres

V477: 8 peaks

B531: 8 peaks

Y595: 8 peaks

R670: 6 peaks

R710: 5 peaks

R792: 4 peaks

 Fluorescence rCV CV < 10% (using QC Fluorospheres at 1 µL/min)
Carryover between Samples 1%a
 Acquisition speed Maximum electronic acquisition speed 16000 events/second with ≤ 95% yield
Recommended maximum sample acquisition speed 5000 events/second to avoid possible swarming or coincidence situations.
 Volumetric Counting accuracy > 90%b

Learn more about nanoscale flow cytometry

a. Tested with polystyrene nanoparticles

b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1µL/min, 2µL/min, 3µL/min, 4µL/min, 5µL/min, 6µL/min respectively, repeat 5 times, then calculate the average total events for each speed, then compare the calculated volumetric counting accuracy with the theoretical total events ( the known concentration 1.3*10 8 x volume).

*polystyrene when triggering on violet side scatter
** In development. Performance characteristics have not been validated.

For Research Use Only. Not for use in diagnostic procedures.