Manutenção de células

Mantenha suas células em um ambiente sem estresse

O processo de semear-cultivar-realizar as passagens-coletar é muitas vezes uma tarefa cotidiana exigida por todos os fluxos de trabalho de cultura celular. Maior consistência com esterilidade, manipulação e manutenção garante reprodutibilidade e repetibilidade dia a dia, laboratório a laboratório, usuário final a usuário final. As soluções automatizadas de cultura celular do Biomek geram resultados confiáveis enquanto otimiza o fluxo de trabalho de cultura de células reduzindo o seu tempo na bancada de trabalho.

Cell Maintenance Resources

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Title	Description
Automated Cell Culture: Improving the Consistency of Cell Maintenance {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	In this application note, we illustrate the capabilities for automating a standard workflow for cell line maintenance and demonstrate this process using mouse embryonic stem cells.
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
Using Cell Impermeant DNA Binding Dyes for Viability Assessment in Multicolor Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.
Automation of Multi-Day Cellular Applications {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Here we demonstrate the use of SAMI Process Management software to schedule and execute the automated liquid handling steps on a Biomek SAMI EX Workstation, for overlapping 8-day embryonic stem cell differentiation experiments.
Manutenção de células
Cellular Solutions of all Shapes and Sizes {FBC52A26-B43E-46C3-A091-65AAEFBE5885}	Beckman Coulter's Mike Kowalski, Ph.D., discusses cell culture challenges and the spectrum of automated solutions for addressing those challenges

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