Monitoramento imunológico

Assistência a pesquisadores para resolver questões em imunologia celular

O monitoramento das respostas imunes celulares é um pré-requisito para o desenvolvimento de vacinas contra o câncer. Os principais objetivos monitoramento imunológico incluem determinar a eficácia de uma vacina para induzir ou aumentar uma resposta específica de células T, bem como o papel que as células T reguladoras (Tregs) - um pequeno subconjunto de células supressoras - têm no desenvolvimento de respostas de células T.

Oferecemos uma variedade de ferramentas e reagentes de monitoramento imune de alta qualidade e tecnologias de ciências da vida projetadas para ajudar os pesquisadores a melhorar sua compreensão do sistema imunológico, das células B e T às vias moleculares.

Immune Monitoring Resources

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Title Description
Assessing T Cell Subpopulations by TCR Expression in PBMCs Application Note
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We outline a protocol using DURAClone™ IM panels, which are unitized, dry format, room temperature stable reagent cocktails, to delineate and quantify T cell subpopulations from a heterogeneous sample of isolated normal PBMCs based on TCR composition.
Using the Separation Quotient Parameter on the AQUIOS CL Flow Cytometer
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Separation Quotient (SQ) is a mathematically calculated parameter that is specific to the AQUIOS CL flow cytometer
MoFlo™ Viability and Function with High-Speed Sorting
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Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
HIV Patient Management Solutions
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The AQUIOS CL Load & Go flow cytometer is an all-in-one solution that incorporates automated loading, sample preparation, reagent management, barcode scanning, data analysis, and full LIS connectivity
AQUIOS Tetra Application Gating Strategy
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The AQUIOS CL flow cytometer is intended for use as a Single Platform Technology (SPT) for lymphocyte subset analysis and enumeration
Instrument Manager Version 8.05 with Bidirectional LIS Connectivity for FC 500 Systems
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Imagine your flow cytometer automatically downloading test requests and patient demographics from the LIS. No need to enter the data manually
Daily Quality Control on the AQUIOS CL System for the Tetra Application
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Flow cytometry, especially immunophenotyping by flow cytometry, is used routinely in the diagnosis prognosis and monitoring of disease
Comparison of Aquios Tetra and Navios Tetra System Performance
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Authors: Violetta Headley, Michael Keeney, Dominika Benjamins, Justin Rohrbach, Robert Ortega, Karen Lo, Liliana Tejidor and Elena Afonina
Long Term Stabilization of Tandem Dyes for Use in High Content, Multi Variant Flow Cytometry
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Authors: Snehita Sattiraju, Tewfik Miloud, Neha Girish, Murthy Pendyala, Sridhar Ramanathan | Beckman Coulter India Pvt Limited, Bangalore Development Centre, Bangalore, INDIA and Beckman Coulter Life Sciences, Immunotech, Marseille, FRANCE
Advanced Analysis of Human T Cell Subsets on the CytoFLEX Flow Cytometer using a 13 Color Tube Based on DuraClone Dry Reagent Technology
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Authors: Nicole Weit, Michael Kapinsky, and Andreas Böhmler | Beckman Coulter GmbH, Krefeld, Germany and Beckman Coulter Immunotech S.A.S, Marseille, France | Published 2015
Comparing the AQUIOS CL PLG Application to the FC500 MCL FlowCARE PLG Application
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The performance of the AQUIOS PLG System (or application) was assessed by comparing the results to the comparator estimation of difference as described in CLSI EP9-A3 Measurement Procedure Comparison and Bias Estimation Using Patient Samples; Approved Guideline, Third Edition.
Error Prevention on the AQUIOS CL System for the Tetra Application
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Laboratories follow procedures defined by laboratory standards and regulations set forth by regulatory bodies such as the Centers for Disease Control (CDC) among others
Beckman Coulter Care Solutions Cares Systems Bring HIV/AIDS Monitoring to a Higher Level of Automation
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By focusing on innovative, yet affordable solutions for HIV monitoring, Beckman Coulter supports the UNAIDS 90-90-90 target to ensure that by the year 2020, 90% of people living with HIV will know their HIV status
Comparison of Aquios Tetra and Navios Tetra System Performance
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Authors: Violetta Headley, Michael Keeney, Dominika Benjamins, Justin Rohrbach, Robert Ortega, Karen Lo, Liliana Tejidor and Elena Afonina | Clinical Application Development, Life Sciences, Beckman Coulter, Inc London Health Sciences Centre, London, Ontario, Canada Clinical Affairs, Diagnostics Beckman Coulter, Inc
Evaluation of the Analytical Performance of the Aquios CL Flow Cytometer in a Multicenter Study
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Authors: Diana Careaga, Robert Magari, Karen Lo, Michael Keeney, Ben Hedley, Dominika Benjamins, Dr. You-Wen Qian, Larry Hinson, Cristina Ceja, Alexandra Amador Casiana Fernandez-Bango, Rosa Colon, Oilda Rubio, Eileen Landrum, Justin Rohrbach, Robert Ortega, Ana Vieira, Dawn Holmes, Raquel Cabana, Pedro Diaz and Liliana Tejidor | Beckman Coulter Diagnostics, Inc., Clinical Research, Miami, Florida, London Health Sciences Centre, London, Ontario, Canada, The University of Texas Medical Branch, Galveston, Texas, University of Miami Miller School of Medicine, Miami, Florida
PerFix-nc (No Centrifuge Assay): A New Intracellular No-Wash Assay for Whole Blood Analysis
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Authors: Fabrice Malergue, Valerie Mallet, Laeticia Khemici, Caroline Scifo, Sylvain Monseaux, Tewfik Miloud, Emmanuel Gautherot, Felix A. Montero-Julian | Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France
Demonstration of the Analytical Performance of the Navios Flow Cytometer in a Multi-Center Study
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Authors: Diana Careaga, Robert Magari, Karen Lo, Michael Keeney, Janice Popma, Joanne Luider, Aito Ueno, Gerard Lozanski, Bruce Briggs, Elena Afonina, Enrique Rabellino and Liliana Tejidor
SWOFF - The Unrecognized Sibling of FMO
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Author: Michael Kapinsky - Beckman Coulter Intl.
Simple and Robust Whole Blood Staining Procedure using PerFix-nc Reagents Generates Unprecedented FOXP3 Signal/Noise Ratio
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Authors: Fabrice Malergue, Laeticia Khemici, Tewfik Miloud, Carlos A. Garcia, Félix A. Montero-Julian | Beckman Coulter Life Science, Inc., Global Assay and Applications Development, Marseille, France, Life Science Research, Miami, USA
Designing Multi-Color Panels for T/Treg Populations using FITMaN Guidelines: a Case Study
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Authors: James W Tung, Jonel Lawson, Carlos A Garcia, Jeffrey R Cobb, Yetty Irwan, William Godfrey, Michael Reed, Enrique Rabellino | Beckman Coulter Inc, Cellular Analysis Business Group, Scientific Affairs, Miami FL
FoxP3+ Tregs Cells Sensitivity to Low Doses of IL-2 Can be Rapidly and Easily Monitored by p-STAT-5 Detection in Ex-Vivo Whole Blood Samples
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Authors: Fabrice Malergue, Valarie Mallet, Caroline Scifo, Tewfik Miloud, Felix A. Montero-Julian | Beckman Coulter Life Sciences, Inc. ; Global Assay and Applications Development, Marseille, France
CytoFLEX Instrument Evaluation Using Biological Specimens
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Authors: James Tung, Dan Condello, Albert Donnenberg, Erika Duggan, Jesus Lemus, John Nolan, Kathy Ragheb, Jennifer Sturgis, Paul Scibelli, J Paul Robinson, Domenic Fenoglio | Beckman Coulter, Miami FL, Purdue University Cytometry Laboratories, La Jolla Bioengineering Institute, University of Pittsburgh Cancer Center
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung - Beckman Coulter, Miami FL
Flow Cytometric Analysis of Multiple Signaling Pathways in LPS Stimulated Human Peripheral Blood Monocytes
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: T Shankey,Sue Chow, and David Hedley | Advanced Technology Group, Beckman Coulter, Inc., Miami, FL, USA , Division of Applied Molecular Oncology, Princess Margaret Hospital, Univ of Toronto, Toronto, Ont., Canada
Measurement of ZAP- 70 Expression in CLL using an Optimized Flow Cytometric Assay for ZAP-70 Protein Levels in Whole Blood Samples
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ZAP-70 protein plays a critical role in the initiation of T-cell signaling through the TCR and also regulates B cell differentiation
Krome Orange™: A Novel Orange-Emitting Dye for Use in Multicolor Flow Cytometry Panels Using Violet Diode Laser Excitation
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Authors: Felix Montero, Ravinder Gupta, Hashem Akhavan-Tafti, Robert Eickholt, Mark Sandison, Rhonda Federspiel, Laura Nieto Gligorovsky, Franck Gaille, Jeffrey Cobb and Emmanuel Gautherot | Beckman Coulter, Inc., Miami, FL, USA; Detroit, MI, USA; Marseille, FRANCE
CD6: New Low/Negative Surface Marker for Human FOXP3+ Naturally-Occurring Regulatory T-Cells
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: Carlos A. Garcia Santana M.D., Ph.D., James W. Tung Ph.D. and Sergei Gulnik Ph.D. | Life Science Research; Life Science Development, Beckman Coulter, Inc., Miami, FL., USA
Drift Analysis Approach to Investigate the Effect of Specimen Age and Prepared Sample Age on the Stability of Lymphocyte Subsets
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Authors: Robert Magari, Jin Zhang, Samantha Bauchiero, Violetta Headley, Liliana Tejidor, and Elena Afonina | Clinical Applications Development, Life Sciences, Beckman Coulter, Inc. - Clinical Research, Diagnostics, Beckman Coulter, Inc.
Beckman Coulter Cares Award- Panel of Judges
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WE CARE THAT YOU CARE
Beckman Coulter Cares Award - Key Dates
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The annual cycle of The Beckman Coulter CARES Award begins in the World AIDS Day 2016, 1st December with a ‘Call for Nominations’
Beckman Coulter Cares Award
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How many years has the nominee been working on this endeavor?
FC 500 Flow Cytometer Series
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Lasers • Uniphase Argon ion, 488nm, 20mW output • Coherent Red Solid State Diode, 635nm, 25mW output
The Beckman Coulter Cares Initiative
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By focusing on innovative, yet affordable solutions for HIV monitoring, Beckman Coulter supports the UNAIDS 90-90-90 target to ensure that by the year 2020, 90% of people living with HIV will know their HIV status
Room Temperature Stable Unitized Reagents for High Content, High Throughput Flow Cytometry Applications
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Authors: Neha Girish, Jean-Marc R Busnel, Samee Saif, Nathalie Dupas, Tewfik Miloud,Vishakha Mangale, Emmaunel Gautherot, Sridhar Ramanathan
An Automated Cell Preparation Method for FlowCARE™ Pan-Leukocyte Gating (PLG) CD4 Assay in a 96-Well Plate Offers Increased Throughput and Ease of Use
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: Jin Zhang, Valentin Quesada, Juan De Castro, Jennifer Zawislak, Jorge Acevedo, Ed Jachimowicz, and Liliana Tejidor | Beckman Coulter, Inc., Cellular Analysis Business Group, Miami, FL
Designing “Backbone” Multi-Color Panels for T-cell and Treg Populations with Allowance for Drop-In Reagents
{E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
Authors: William L. Godfrey, Jonel G. Lawson, Carlos A. Garcia, Enrique Rabellino, Jeffrey R. Cobb, and James W. Tung
Beyond Matching Dim Expression Densities with Bright Fluorochromes A Novel Practical Approach to Design Multicolor Applications
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Author: Michael Kapinsky, Beckman Coulter Life Science, Inc., Krefeld, Germany
Enumeration of T, B and NK Subpopulations in Aged Whole Blood Samples using AQUIOS CL Instrument with Tetra Application
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
Authors: Violetta Headley, Xiangyang Dong , Raquel Cabana , Veronica Trujillo , Michael Thomas, Robert Magari, Elena Afonina | Clinical Application Development, Life Sciences, Beckman Coulter, Inc Research, Life Sciences Beckman Coulter Inc. Clinical Affairs, Diagnostics, Beckman Coulter, Inc
DuraClone Improves Standardization of Compensation Workflow for High Content Multicolor Applications by Flow Cytometry
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Multiparametric flow cytometry is a valuable tool for clinical research as it allows the use of multiple dyes, including tandems, to monitor several markers simultaneously
Competitive Mouse Bone Marrow Transplantation Reconstitution Assay Application Note
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Using primary-conjugated antibodies against CD45.1 and CD45.2, we measure competitive bone marrow transplantation success by quantifying the proportion of donor cells (marked by CD45.1/Ly5.1 expression) and recipient cells (marked by CD45.2/Ly5.2 expression) in the peripheral blood of irradiated recipient mice post-transplantation.
A High Recovery and High Purity Data Analysis Strategy for Rare Abnormal Plasma Cell Events using the DuraClone RE PC Tube
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Authors: Dr Agnieszka Blum- Charité Virchow Hospital, Stem Cell Facility, Berlin, Germany | Dr Michael Kapinsky - Life Science, Beckman Coulter, Marseille, France
Phenotypic Characterization of Human Natural Killer Cell Populations in Peripheral Blood Application Note
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Describes a 10 color, 13 marker panel to investigate the NK cell population, both phenotypically and functionally in human PBMC samples. The panel includes the use of an exclusion markers on a dump channel used to gate out non-NK cells. Deep phenotyping markers for the NK cell compartment are included and a comprehensive gating strategy is defined.
Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System
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Authors: William L. Godfrey, Li Yang, Samantha Bauchiero, James W. Tung, Valerie Mallet, Lidice Lopez, Vincent Shankey, Emmanuel Gautherot | Beckman Coulter Inc, Miami, FL, USA; Marseille, FRANCE
Use of Precision Profiles to Estimate Precision of Lymphocyte Subset Analysis by Flow Cytometry
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Authors: Robert Magari, Diana, B. Careaga, Karen, K. Lo, Xiangyang Dong, Elena Afonina, Liliana, M. Tejidor
Beckman Coulter Cares Award- Your Frequently Asked Questions
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The Beckman Coulter CARES Award seeks to honour today’s heroes and heroines
Demonstration of the Analytical Performance of the Navios Flow Cytometer in a Multi-Center Study
{E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
Authors: Diana Careaga, Robert Magari, Karen Lo, Michael Keeney, Janice Popma, Joanne Luider, Aito Ueno, Gerard Lozanski, Bruce Briggs, Elena Afonina, Enrique Rabellino and Liliana Tejidor
University of Texas Medical Branch (UTMB) Workflow Comparison Study with the Aquios CL Flow Cytometer
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Many traditional flow cytometry systems, such as the current Becton Dickinson FACSCalibur™ with SPA II, require either manual or semi-automated preparation of samples, manual creation of worklists, manual data review, and manual tabulation of numerical data
Beckman Coulter Cares Award - Rules and Eligibility
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The Cares Award (“Award”) will consist of a $5000 donation, made in the name of the recipient of the Award, to a charitable organization chosen by the recipient of the Award from a pre-defined list
London Health Sciences Centre (LHSC) Workflow Comparison Study with the Aquios CL Flow Cytometer
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The flow cytometry laboratory at Victoria Hospital is situated within a tertiary care academic institution, London Health Sciences Centre
Enumeration of T, B, and NK Subpopulations in Aged Whole Blood Samples using the Aquios CL Instrument with Tetra Application
{E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
Authors: Violetta Headley, Xiangyang Dong, Raquel Cabana, Veronica Trujillo, Michael Thomas, Robert Magari and Elena Afonina
Evaluation of the Analytical Performance of the Aquios CL Flow Cytometer in a Multi-Center Study
{E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
A multi-center study was conducted to establish the performance of the AQUIOS CL Flow Cytometer with AQUIOS Tetra Panel reagents for lymphocyte subset analysis
Flow Cytometric Analysis of Endothelial Progenitor Cells
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Authors: Dorota Sadowicz, Vasilis Toxavidis, John Tigges | Beth Israel Deaconess Medical Center | Harvard Stem Cell Institute
Comparing the Aquios CL Flow Cytometer, the Facscalibur Flow Cytometer and the Unicel DXH 800 Hematology Analyzer Tetra Applications
{E588CF40-730C-4137-A5DC-8DDEAB7CF7D9}
The comparability of the AQUIOS Tetra System was assessed by comparing the results with the predicate method using the estimation of difference as described in CLSI EP9-A3 Method Comparison and Bias Estimation
Monitoramento imunológico

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