Purificação de PCR

O sistema AMPure XP da Agencourt é um sistema de purificação de PCR altamente eficiente e facilmente automatizado que fornece DNA de qualidade superior sem salinidade. Sem necessitar de centrifugação ou de filtragem, o Agencourt AMPure XP pode ser facilmente utilizado em formatos manuais e automáticos de 96 ou 384 poços.

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AMPure XP Workflow

Product Features

Overview

AMPure XP removes unwanted contaminants from DNA in a variety of applications including PCR, NGS, cloning, and microarrays.

High Recovery

Efficient recovery of your sample ensures that critical data is not lost within the workflow.

Gold Standard for NGS Workflows

Trusted and recommended reagent for size selection and cleanup steps within a variety of NGS workflows.

Flexible and Scalable

Manual cleanups using AMPure XP require less than 30 minutes. For higher throughput needs, AMPure XP is automated on all Biomek platforms enabling hundreds of samples per day.

Citations

Peffers, M. J., Liu, X., & Clegg, P. D. (2014, March 8). Transcriptomic profiling of cartilage ageing. Genomics Data, 2, 27-28. doi:10.1016/j.gdata.2014.03.0011

  • Used to clean up cDNA

Greenwald, W. W., Li, H., Benaglio, P., Jakubosky, D., Matsui, H., Schmitt, A., . . . Frazer, K. A. (2019, March 5). Subtle changes in chromatin loop contact propensity are associated with differential gene regulation and expression. Nature Communications, 10(1054), 1-17. doi:https://doi.org/10.1038/s41467-019-08940-51

  • Used to clean up barcoded PCR reactions during Library preparation

Don't Lose Critical Data

When so much more has been invested in your research, AMPure XP is the only choice for purification and clean-up steps. Loss of yield during this critical step leads to loss of discovery in your research.

Relative costs of the different steps required to perform various NGS applications. Steps include Extraction, Library Construction, Library Enrichment, Clean-up, and Sequencing. Costs were calculated based on average list price of commercially available kits and reagents in 2017. Clean-up efficiencies were calculated by determining the total DNA yield by Picogreen Assay after performing a clean-up procedure on a known amount of DNA. The percent yield relative to AMPure XP performance was then used to calculate the impact of efficiency on various commercially available library construction methods and a change in purification reagent.

AMPure XP - Keep Critical Data For Downstream Applications

AMPure XP is the Gold Standard for Bead Based, Next-Generation Sequencing (or NGS) Cleanup

Over 200 library prep kits suggest using AMPure XP, including kits from trusted sequencing companies:

  • Illumina®
  • Nanopore
  • PacBio
  • Thermo Scientific

Over 15,000 publications used AMPure XP, referenced in articles in Science, Nature, and PNAS.

Nucleic acid purification and cleanup are mandatory for genomic applications, such as sequencing, qPCR/ddPCR/PCR, and microarrays. Maximizing recovery, consistency, and speed, AMPure XP meets the stringent needs of today’s genomic applications and minimizes the risk of losing important genetic information.

  • High recovery of amplicons greater than 100bp
  • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
  • Predictable and consistent size selection

“Our findings suggest AMPure XP would be the best choice for analyses requiring very high analytical stringency.” Mikheikin, A., Olsen, A., Picco, L. et al. High-speed atomic force microscopy revealing contamination in DNA purification systems. Anal. Chem. 88:5, 2527-2523 (2016) doi: 10.1021/acs.analchem.5b04023.1

1Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Biomek Automated Genomic Sample Prep Accelerates Research Biomek i-Series Automated Beckman Coulter AMPure XP PCR Purification System The Beckman Coulter AMPure XP kit is a highly efficient PCR purification system that delivers
Datasheet - EMnetik System The EMnetik 24 microparticle processor is a semi-automated device designed for PCR cleanup and plasmid purification
Customer Spotlight with Bi Lab Studying how the biochemical and transcriptional regulation and evolution of myomaker and myomixer drive muscle development, Dr. Bi and his team evaluate the newly developed EMnetik 24 microparticle processor system.
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.

Documentos Técnicos